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Determination of diffusion coefficients by spreading from thin layers
Author(s) -
Polson A.,
Parkyn D. G.
Publication year - 1969
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1969.360070111
Subject(s) - chemistry , diffusion , tube (container) , thin layer , diffusion layer , thin film , layer (electronics) , convection , thin layers , analytical chemistry (journal) , chromatography , composite material , mechanics , thermodynamics , nanotechnology , materials science , organic chemistry , physics
Abstract Two new methods for the measurement of diffusion coefficients were introduced. In the first method the spreading is measured from a. thin layer in a cylindrical tube containing a suitable gel. To avoid convection in the gel the tube is rotated horizontally round its long axis. In the second method the diffusion spreading is measured from an initial thin layer under a column of solvent in a Tiselius electrophoresis cell or a micro cuvet of a spectrophotometer. The great advantage is the economy on the material investigated.