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Reinvestigation of the primary structure of yeast alanine tRNA
Author(s) -
Merril Carl R.
Publication year - 1968
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1968.360061207
Subject(s) - alanine , transfer rna , chemistry , yeast , protein primary structure , biochemistry , rna , guanine , stereochemistry , peptide sequence , amino acid , nucleotide , gene
The primary structure of alanine transfer RNA reported by Holley and his colleagues contained a number of inconsistencies between the data and the proposed sequence. In order to resolve these inconsistencies, alanine tRNA was isolated from yeast by using countercurrent techniques similar to those employed by Holley. The alanine accepting RNA obtained by this procedure was found by further purification employing reverse phase partition chromatography, to contain two different species of alanine tRNA. One of these, tRNA ala I, could be shown to be similar to Holley's proposed structure with the addition of a guanine between positions 47 and 48 and only 2 dihydrouridines instead of the 2.5 reported by Holley.