Premium
Interchain hydrogen bonds via bound water molecules in the collagen triple helix
Author(s) -
Ramachandran G. X.,
Chandrasekharan R.
Publication year - 1968
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1968.360061109
Subject(s) - chemistry , hydrogen bond , molecule , crystallography , triple helix , triple bond , hydrogen , helix (gastropod) , stereochemistry , collagen helix , double bond , polymer chemistry , organic chemistry , ecology , snail , biology
Abstract If the collagen triple helix is so built as to have one set of NH ⃛ O hydrogen bonds of the type N 3 H 3 (A) ⃛ O 2 (B), then it is possible to have a linkage between N 1 H 1 (B) and O 1 (A) through the intermediary of a water molecule with an oxygen O 1 Wleading to the formation of the hydrogen bonds N 1 (B) ⃛ O 1 Wand O 1 W(A). In the same configuration, another water molecule with an oxygen O 1 Wcan link two earbonyl oxygens of chains A and B forming the hydrogen bonds O 2 WO 1 (A) and O 2 WO 0 (B). The two water oxygens also become receptors at the same time for CH ⃛ O hydrogen bonds. Thus, the neighboring chains in the triple helix are held together by secondary valence bond linkages occurring regularly sit intervals of about 3 Å along the length of the protofibril. The additional water molecules occur on the periphery of the proto‐fibril and will contribute their full share towards stabilizing the structure in the solid state. In solution, they will be disturbed by the medium unless they are protected by long side groups. It appears that this type of two‐bonded structure, in which one NH ⃛ O bond is to a water molecule, can explain several observations on the stability and hydrogen exchange properties of collagen itself and related synthetic polypeptides. The nature of the water bonds and their strength are found to be better in the one‐bonded structure proposed from Madras than in the one having the coordinates of Rich and Crick.