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Molecular weight of T7 and calf thymus DNA by low‐angle light scattering
Author(s) -
Harpst J. A.,
Krasna Alvin I.,
Zimm B. H.
Publication year - 1968
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1968.360060413
Subject(s) - extrapolation , chemistry , scattering , dna , light scattering , molecular mass , molecular physics , crystallography , optics , physics , biochemistry , mathematics , enzyme , mathematical analysis
A low‐angle light‐scattering instrument has been used to measure molecular weights of native calf‐thymus and T7 DNA. Molecular weights obtained by extrapolation of angular data to 0° from measurements above 30° are less than molecular weights from extrapolation of data taken at low angles (below 30°). The low‐angle molecular weights determined for calf‐thymus DNA and for T7 DNA agree well with estimates of weight‐average molecular weight obtained with other techniques. The low‐angle light‐scattering molecular weight for calf‐thymus DNA is higher than previously reported values by light scattering at angles above 30°. A concentration dependence in the scattering from DNA solutions is also observed.