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Low‐angle light‐scattering instrument for DNA solutions
Author(s) -
Harpst J. A.,
Krasna Alvin I.,
Zimm B. H.
Publication year - 1968
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1968.360060412
Subject(s) - optics , calibration , scattering , light scattering , multiangle light scattering , interference (communication) , beam (structure) , chemistry , light beam , light source , sample (material) , measuring instrument , forward scatter , physics , computer science , computer network , channel (broadcasting) , chromatography , quantum mechanics , thermodynamics
A light‐scattering instrument capable of measurements on native DNA at angles as low as 10° is described. The major features of the instrument, which make it capable of low‐angle measurements, are the use of an intense light source in which the incident beam is monitored directly and the use of a long, rectangular sample cell in which the scattered light can be measured at low angles with no interference from the incident beam. Methods for calibrating the instrument and for determining scattering correction factors are described. Procedures for the preparation and use of various calibration standards are given.