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Sedimentation coefficients of replicating and crosslinked DNA
Author(s) -
Bloomfield Victor A.
Publication year - 1968
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1968.360060303
Subject(s) - sedimentation coefficient , sedimentation , chemistry , dna , function (biology) , volume (thermodynamics) , replication (statistics) , crystallography , analytical chemistry (journal) , thermodynamics , chromatography , physics , geology , statistics , mathematics , geomorphology , biochemistry , sediment , genetics , biology , enzyme
The sedimentation coefficients of branched and cyclic replicating DNA structures have been calculated taking into account both chain stiffness and excluded volume effects. For branched DNA, the calculated ratio S 0 (α)/ S 0 (0) of the sedimentation coefficient at degree of replication α to that at α = 0 increases from 1.000 to 1.331 as α goes from 0 to 1. For cyclic replicating DNA, S 0 (1)/ S 0 (0) = 1.517. The considerable difference between these two ratios for α = 1 suggests that the two types of replicating structures may feasibly be distinguished by sedimentation velocity studies, A similar calculation has been made of the sedimentation coefficient of singly cross‐linked DNA under denaturing conditions, as a function of placement of the crosslink. In 0.195 M Na + , the ratio S 0 (α)/ S 0 (0) varies from 1.000 at α = 0 to 1.124 at α = 0.5, where α is the fractional distance of the crosslink from the end of the chain.