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Infrared studies of deoxyribonucleic acids, their constituents and analogues. II. Deoxyribonucleic acids with different base composition
Author(s) -
Fritzsche H.
Publication year - 1967
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1967.360050908
Subject(s) - chemistry , cytosine , guanine , dna , nucleic acid , infrared spectroscopy , gc content , nucleobase , infrared , polynucleotide , ap site , base (topology) , biochemistry , analytical chemistry (journal) , chromatography , nucleotide , organic chemistry , dna damage , genome , mathematical analysis , physics , mathematics , optics , gene
The base composition of DNA from microorganisms varies over a wide range of guanine + cytosine (G‐C) content. We have examined the infrared spectra of a number of DNA samples isolated from different microorganisms and have found marked differences in their infrared spectra. These differences have been related to the G‐C content of the investigated DNA samples. A comparison between the infrared spectra of nucleosides, synthetic polynucleotides, apurinic acid, and apyrimidinic acid has permitted a more extensive assignment of infrared bands to distinct base residues. The relative intensity of the absorption bands at 1485 and 1505 cm. −1 depends on the G‐C content of the deuterated DNA sample. On the basis of these results a new method for the determination of the G‐C content in DNA has been developed from the results. Its advantage is that the measurement is rapid and the DNA samples are not changed by the procedure.