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Derivatives of proteins. II. Coupling of α‐chymotrypsin to carboxyl‐containing polymers by use of N ‐ethyl‐5‐phenylisoxazolium‐3′‐sulfonate
Author(s) -
Patel R. P.,
Lopiekes D. V.,
Brown S. P.,
Price S.
Publication year - 1967
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1967.360050611
Subject(s) - chemistry , sulfonate , chymotrypsin , polymer , covalent bond , amine gas treating , yield (engineering) , organic chemistry , conjugate , polymer chemistry , enzyme , trypsin , sodium , mathematical analysis , materials science , mathematics , metallurgy
A method for covalently binding proteins to carboxyl‐containing polymers is described. N ‐Ethyl‐5‐pheriylisoxazolium‐3′‐sulfonate is used to form active esters of the carboxyls, which then condense with amine groups on the protein to yield amides. Conjugates of α‐chymotrypsin with such polymers have been obtained, with nearly full retention of enzymatic activity in some preparations.