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Spectrophotometric titration studies on poly (uridylic acid)
Author(s) -
Simpkins H.,
Richards E. G.
Publication year - 1967
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1967.360050609
Subject(s) - chemistry , titration , ionization , uracil , uridine , salt (chemistry) , acid dissociation constant , inorganic chemistry , crystallography , rna , biochemistry , aqueous solution , ion , organic chemistry , gene , dna
In order to provide preliminary data for the interpretation of the spectrophotometric titration properties of RNA, the spectral changes accompanying the ionization of poly‐(uridylic acid) have been determined, as have the ionization constants as a function of salt concentration and the enthalpies and entropies of ionization. The spectral propertics and ionization constants of poly (uridylic acid) have been compared with those of 2′(3′) Uridine monophosphate and of uridine; significant, differences have been established. The results obtained are consistent with the hypothesis that uracil residues in poly U are stacked only in concentrated salt solutions.