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Base composition of intact nucleic acid oligomers
Author(s) -
Mandeles Stanley,
Cantor Charles R.
Publication year - 1966
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1966.360040706
Subject(s) - oligomer , chemistry , dimer , nucleic acid , oligonucleotide , composition (language) , ribonuclease , rna , urea , pancreatic ribonuclease , tobacco mosaic virus , crystallography , polymer chemistry , biochemistry , organic chemistry , dna , virus , linguistics , philosophy , virology , biology , gene
The base composition of intact, purified, oligonucleotides has been determined by comparing the absorption spectrum of each oligomer with calculated curves. The spectral curve of each oligomer was measured in 7 M urea at three pH values and digitized at 1‐mμ intervals. The calculated curves consisted of the sums of the absorption spectra of mononucleotides in 7 M urea at the same pH's. A computer was used to make the comparisons and establish the best fit. Results are presented for nine of the ten possible dimer composition isomers; trimers from pancreatic and T 1 ribonuclease hydrolyzates of TMV‐RNA; poly‐A, poly‐C, and poly‐U; and intact tobacco mosaic virus‐RNA.