Sedimentation coefficient of T‐even bacteriophage DNA | Zendy

Rosenbloom J. | Zendy; Cox E. C. | Zendy

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Sedimentation coefficient of T‐even bacteriophage DNA

Author(s) -

Rosenbloom J.,

Cox E. C.

Publication year - 1966

Publication title -

biopolymers

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.556

H-Index - 125

eISSN - 1097-0282

pISSN - 0006-3525

DOI - 10.1002/bip.1966.360040705

Subject(s) - centrifuge , sedimentation coefficient , sedimentation , chemistry , dna , centrifugation , bacteriophage , coefficient of variation , chromatography , microbiology and biotechnology , analytical chemistry (journal) , biochemistry , geology , sediment , physics , biology , geomorphology , enzyme , gene , escherichia coli , nuclear physics

The sedimentation coefficient of T2 phage DNA has been studied by zone centrifugation in sector‐shaped preparative centrifuge tubes over a concentration range of 0.02–2.0 μg./ml. DNA. These results have been compared to a similar study in the analytical centrifuge of T4 DNA over the range of 0.50–5.75 μg./ml. DNA. A value for the sedimentation coefficient of 60.7 ± 1.8 S. was obtained by the first method and a value of 61.3 ± 1.5 S. by the second.

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