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Some water‐insoluble papain derivatives
Author(s) -
Silman Israel H.,
AlbuWeissenberg Martin,
Katchalski Ephraim
Publication year - 1966
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1966.360040405
Subject(s) - papain , chemistry , covalent bond , salt (chemistry) , water soluble , solubilization , enzyme , copolymer , organic chemistry , monomer , chromatography , biochemistry , polymer
A description is given of the preparation and properties of four water‐insoluble papain derivatives by three different techniques: ( a ) covalent binding to a water‐insoluble diazoimitn salt derived from a copolymer of p ‐aminophenylalanine and leucine; ( b ) binding to insoluble preparations of collagen via bisdiazobenzidine‐2,2′‐disulfonic acid; ( c ) in‐solubilization by crosslinking the enzyme itself with bisdiazobenzidine. By all three methods it, was possible to obtain stable water‐insoluble papain derivatives retaining much of the activity of the native enzyme on low and high molecular weight substrates.