Premium
Study of biological activity regenerated by the oxidation of fully reduced insulin
Author(s) -
Pruitt Kenneth M.,
Robison Bonnie S.,
Gibbs Julian H.
Publication year - 1966
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1966.360040308
Subject(s) - chemistry , insulin , yield (engineering) , thiosulfate , urea , metal , catalysis , biochemistry , organic chemistry , medicine , sulfur , materials science , metallurgy
Various techniques for recombining the fully reduced A and H chains of insulin were studied. Best results were obtained from water solutions of the reduced chains allowed to oxidize by standing in air at 3 °C. and from metal‐catalyzed oxidation under the same conditions but in 7.6 M urea solutions. Average net regeneration of insulin was 6% by weight, based on rat hemidiaphragm analysis for insulin activity. The theoretical yield for a random recombination of the A and B chains was calculated to be 3%. The 50% yield of insulin (from the oxidation of reduced insulin thiosulfate) reported by Jiang et al. could not. be confirmed.