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Optical rotatory dispersion of egg proteins. I. Ovalbumin, conalbumin, ovomucoid, and lysozyme
Author(s) -
Tomimatsu Yoshio,
Gaffield William
Publication year - 1965
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1965.360030502
Subject(s) - ovalbumin , chemistry , lysozyme , optical rotatory dispersion , crystallography , analytical chemistry (journal) , chromatography , circular dichroism , biochemistry , immune system , immunology , biology
Optical rotatory dispersions (ORD) of ovalbumin, conalbumin, ovomucoid, and lysozyme (muramidase) were measured in aqueous solution over a wavelength range of 195–600 mμ. The dispersion curves obeyed a one‐term Drude equation above 300 mμ. The strong Cotton effects in the ultraviolet showed peaks and troughs at about the same wavelength for all four proteins, but the crossover point for the 225 mμ Cotton effect for ovomucoid was appreciably lower than for the other proteins. Helical contents calculated by using the Moffitt equation, the trough value of the 225 mμ Cotton effect, and the expressions for helical content recently proposed by Schechter and Blout ranged from 5 to 34%. Except for ovomucoid, the trough value of the 225 mμ Cotton effect gave the lowest helical content.