Premium
The adsorption of polyribonucleotides
Author(s) -
Winsten Walter A.
Publication year - 1964
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1964.360020405
Subject(s) - chemistry , aqueous solution , adsorption , microporous material , van der waals force , counterion , inorganic chemistry , hydrophobic effect , polyethylene , polymer chemistry , organic chemistry , molecule , ion
Using a chromatographic technique, the adsorption of the polyribonucleotides s‐RNA and poly‐A provided with tri‐ n ‐butylammonium ions from an aqueous solution on to the surface of polymeric hydrocarbon granules has been demonstrated. The aqueous solution from which adsorption takes place is the aqueous phase which results on agitating together n ‐butanol (100), water (130), tri‐ n ‐butylamine (10), n ‐butyl ether (10), and glacial acetic acid (2.5) (parts by volume). Microporous polyethylene granules, microporous fluorinated polyethylene granules, and solid grains of polyethylene powder have been employed as adsorbents. The adsorbed polyribonucleotides could be eluted by aqueous solutions of sodium chloride, urea, or triethylammonium acetate. s‐RXA with tri‐ n ‐butylammonium counterions could also be adsorbed on polymethacrylic acid in tri‐ n ‐butylammonium form and eluted therefrom with aqueous urea solutions. It is considered that the adsorption from aqueous solution of polyribonucleotides with tri‐ n ‐butylammonium counterions on polymeric hydrocarbon surfaces is mediated by hydrophobic bond formation. Van der Waals forces may also contribute to the bonding. It is suggested that studies on the adsorption of polynucleotides provided with counterions: possessing nonpolar groups at an aqueous solution‐polymeric hydrocarbon interlace may permit the elaboration of experimental systems to serve as models for the evaluation of the role of hydrophobic and van der Waals bonding forces in the interaction of polynucleotides with cell membrane surfaces.