Premium
Electrophoresis of the nucleic acids
Author(s) -
Olivera Baldomero M.,
Baine Peter,
Davidson Norman
Publication year - 1964
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1964.360020306
Subject(s) - nucleic acid , chemistry , ionic strength , dna , electrophoresis , titration , ionic bonding , rna , analytical chemistry (journal) , chromatography , ion , biochemistry , inorganic chemistry , organic chemistry , aqueous solution , gene
A zone electrophoresis apparatus using ultraviolet, optics has been constructed to study nucleic acids at concentration less than 0.004%. Native DNA has a mobility about 15% higher than denatured DNA over a range of conditions of pH and ionic strength. DNA's from different sources have closely similar mobilities. A study of a molecular weight series of DNA indicates that the mobility is constant in the molecular weight range of 2.5 × 10 5 to 1.3 × 10 8 . DNA mobilities change in the expected way with pH but the fractional change in mobility is less than the change in charge calculated by titration curves. A small decrease in mobility accompanies an increase in ionic strength. RNA's from various sources have mobilities slightly lower than denatured DNA except for s‐RNA which travels slightly faster.