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Nuclear magnetic resonance study of synthetic polynucleotides and transfer RNA
Author(s) -
McTague John P.,
Ross Virginia,
Gibbs Julian H.
Publication year - 1964
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1964.360020208
Subject(s) - polynucleotide , chemistry , transfer rna , denaturation (fissile materials) , ribose , monomer , spectral line , proton , crystallography , nuclear magnetic resonance , rna , stereochemistry , polymer chemistry , biochemistry , polymer , organic chemistry , nuclear chemistry , physics , quantum mechanics , astronomy , gene , enzyme
High resolution proton spectra in D 2 O solutions have been measured as functions of temperature for the following polynucleotides: Poly A, Poly U, Poly A and Poly U, Poly I, Poly C, Poly I and Poly C, and Transfer UNA from E. coli (strain B), and from yeast. At denaturation, peaks characteristic of the mononucleotide protons appear. Areas under the peaks can be used as a direct measure of denaturation. The sharpness of the spectral lines indicates that in the denatured form internal rotation about the ribose‐phosphate and the ribose‐base links is relatively free. Linewidths also show that monomer units remain in either the ordered or freely rotating state for at least 10 −4 sec.