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Exoenzyme kinetics with applications to the determination of nucleotide sequences
Author(s) -
Cantor Charles R.,
Tinoco Igxacio,
Peller Leonard
Publication year - 1964
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1964.360020108
Subject(s) - nucleotide , chemistry , kinetics , dodecameric protein , nucleic acid , sequence (biology) , exoenzyme , nucleic acid sequence , stereochemistry , biochemistry , dna , gene , physics , quantum mechanics , enzyme
The kinetics of the action of exoenzymes on linear polymers is developed. The results are applied to the problem of sequence determination in nucleic acids. It is found that a choice of experimental conditions different from the steady‐state situation provides a liberation of successive nucleotides in better resolved time intervals. The inference of the nucleotide sequence of a hypothetical dodecamer is explored in detail. Calculations indicate the possibility of determining the base sequences of oligomers containing up to 25 nucleotides.