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Targeting duplex DNA with DNA‐PNA chimeras? Physico‐chemical characterization of a triplex DNA‐PNA/DNA/DNA
Author(s) -
Petraccone L.,
Erra E.,
Messere A.,
Montesarchio D.,
Piccialli G.,
De Napoli L.,
Barone G.,
Giancola C.
Publication year - 2004
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10599
Subject(s) - dna , chemistry , duplex (building) , nucleic acid thermodynamics , dna clamp , peptide nucleic acid , microbiology and biotechnology , dna sequencing , base pair , biochemistry , biophysics , rna , biology , gene , base sequence , reverse transcriptase
Targeting double‐stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA‐PNA chimeras. A 16‐mer 5′‐DNA‐3′‐p‐(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all‐DNA complex. © 2004 Wiley Periodicals, Inc. Biopolymers, 2004