Targeting duplex DNA with DNA‐PNA chimeras? Physico‐chemical characterization of a triplex DNA‐PNA/DNA/DNA | Zendy

Petraccone L. | Zendy; Erra E. | Zendy; Messere A. | Zendy; Montesarchio D. | Zendy; Piccialli G. | Zendy; De Napoli L. | Zendy; Barone G. | Zendy; Giancola C. | Zendy

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Targeting duplex DNA with DNA‐PNA chimeras? Physico‐chemical characterization of a triplex DNA‐PNA/DNA/DNA

Author(s) -

Petraccone L.,

Erra E.,

Messere A.,

Montesarchio D.,

Piccialli G.,

De Napoli L.,

Barone G.,

Giancola C.

Publication year - 2004

Publication title -

biopolymers

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.556

H-Index - 125

eISSN - 1097-0282

pISSN - 0006-3525

DOI - 10.1002/bip.10599

Subject(s) - dna , chemistry , duplex (building) , nucleic acid thermodynamics , dna clamp , peptide nucleic acid , microbiology and biotechnology , dna sequencing , base pair , biochemistry , biophysics , rna , biology , gene , base sequence , reverse transcriptase

Targeting double‐stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA‐PNA chimeras. A 16‐mer 5′‐DNA‐3′‐p‐(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all‐DNA complex. © 2004 Wiley Periodicals, Inc. Biopolymers, 2004

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