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Incorporation of anthraquinonyl group into λ‐Cro repressor protein for strand‐ and position‐specific photocleavage of double‐stranded DNA
Author(s) -
Sasaki Hiroshi,
Ikeda Kazumasa,
Suzuki Maya,
Ninomiya Keiko,
Sisido Masahiko
Publication year - 2004
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10564
Subject(s) - chemistry , repressor , dna , mutant , piperidine , escherichia coli , in vitro , sequence (biology) , stereochemistry , biochemistry , gene , transcription factor
λ‐Cro repressor protein incorporated with a 2‐anthraquinonylalanine (anqAla) at the 64th position was chemically synthesized by solid‐phase method. The 64th position was selected according to previous information on various mutants of Cro incorporated with a single anqAla unit, that were synthesized through an Escherichia coli in vitro protein‐synthesizing system. The 64anqAla mutant bound to a dsDNA of consensus operator sequence and underwent strand‐ and position‐specific photocleavage of the dsDNA at the GG sequence after treatment with piperidine. The mutant also underwent position‐specific self‐photoscission. The self‐photoscission was retarded in the presence of the dsDNA. © 2004 Wiley Periodicals, Inc. Biopolymers (Pept Sci) , 2004