Premium
Studies on acute human infections using FTIR microspectroscopy and cluster analysis
Author(s) -
Mordehai Jacov,
Ramesh Jagannathan,
Huleihel Mahmoud,
Cohen Zahavi,
Kleiner Oleg,
Talyshinsky Marina,
Erukhimovitch Vitaly,
Cahana Aviv,
Salman Ahmad,
Sahu Ranjit Kumar,
Guterman Hugo,
Mordechai Shaul
Publication year - 2004
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10554
Subject(s) - fourier transform infrared spectroscopy , cluster (spacecraft) , human blood , whole blood , chemistry , white blood cell , medicine , physiology , optics , physics , computer science , programming language
A novel methodology for the diagnosis of acute infections using FTIR microspectroscopy (FTIR‐MSP) data on blood components and cluster analysis is presented. Blood samples were collected from 11 patients suffering from various infections and 16 age‐matched healthy human controls. Blood components such as white blood cells, red blood cells, and plasma were isolated using standard procedures and FTIR‐MSP of these components was utilized. A cluster analysis of the FTIR spectra was performed. The spectra obtained from the three blood components of patients were different from those of controls. The FTIR spectra of white blood cells from patients suffering infections were significantly different from the controls. Cluster analyses of averaged FTIR‐MSP spectra of white blood cells provided 100% classification between patients and healthy controls. © 2004 Wiley Periodicals, Inc. Biopolymers, 2004