Premium
Spectroscopic study of permanganate oxidation reactions of oligonucleotides containing single base mismatches
Author(s) -
Bui Chinh T.,
Lambrinakos Andreana,
Cotton Richard G. H.
Publication year - 2003
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10543
Subject(s) - chemistry , permanganate , oligonucleotide , base (topology) , dna , derivative (finance) , spectroscopy , photochemistry , inorganic chemistry , biochemistry , mathematical analysis , physics , mathematics , quantum mechanics , financial economics , economics
Abstract The electrophoretic gel‐based chemical cleavage of the mismatch method gives an incomplete view of the DNA conformational changes induced by a single base mismatch. This spectroscopic study investigates the permanganate oxidation reactions with matched and mismatched DNA under constant and variable temperature conditions. The results, which include the oxidation levels, reaction patterns with isosbestic points, color changes, thermal spectra, spectroscopy derivative, and gel separation and melting temperatures, provide a fundamental background for identification of oligonucleotides containing single base mismatches by chemical means. © 2003 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 70: 628–636, 2003