Premium
Fluorescence properties of labeled proteins near silver colloid surfaces
Author(s) -
Maliwal Badri P.,
Malicka Joanna,
Gryczynski Ignacy,
Gryczynski Zygmunt,
Lakowicz Joseph R.
Publication year - 2003
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10501
Subject(s) - chemistry , avidin , monolayer , fluorescence , biotinylation , excitation , molecule , colloid , analytical chemistry (journal) , adsorption , photochemistry , optics , chromatography , organic chemistry , physics , biochemistry , electrical engineering , engineering
The fluorescence properties of a monolayer of labeled avidin molecules were studied near silver island films. We first adsorbed a monolayer of biotinylated‐BSA as a base that was used to capture labeled avidin molecules. For labeled avidin on silver island films, we observed an increase of the fluorescence intensity of between 18 and 80 with one‐photon excitation and up to several hundredfold or larger with two‐photon excitation. The probes were moderately more photostable in the presence of silver islands. There was also a dramatic decrease in the lifetimes with the amplitude‐weighted values decreasing from 7‐ to 35‐fold. The data suggest that these spectral changes are due to both increased rates of excitation near the metallic particles and increases in the rates of radiative decay. Because these silver island surfaces are very heterogeneous, we are hopeful that larger increases in intensity and photostability can be obtained for probes situated at an optimal distance from the ideal island surfaces. © 2003 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 70: 585–594, 2003