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Simple Pb II fluorescent probe based on Pb II ‐catalyzed hydrolysis of phosphodiester
Author(s) -
Sun Ming,
Shangguan Dihua,
Ma Huimin,
Nie Lihua,
Li Xiaohua,
Xiong Shaoxiang,
Liu Guoquan,
Thiemann Wolfram
Publication year - 2003
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10484
Subject(s) - phosphodiester bond , chemistry , fluorophore , fluorescence , photochemistry , phenoxazine , intramolecular force , electron acceptor , acceptor , electron donor , quenching (fluorescence) , photoinduced electron transfer , electron transfer , catalysis , stereochemistry , organic chemistry , medicine , rna , biochemistry , physics , quantum mechanics , phenothiazine , pharmacology , gene , condensed matter physics
Abstract A new fluorescent probe for Pb II , p ‐nitrophenyl 3 H ‐phenoxazin‐3‐one‐7‐yl phosphoric acid (NPPA), was designed and synthesized by linking resorufin (serving as a fluorophore and electron acceptor) to p ‐nitrophenol (serving as a fluorescence quencher and electron donor) through phosphodiester bonds. When NPPA was irradiated with light, intramolecular fluorescence self‐quenching took place because of the photoinduced electron transfer from the donor to the acceptor. However, upon the addition of Pb II , the phosphate ester bonds in the probe were cleaved and the fluorophore was released, accompanying the retrievement of fluorescence. © 2003 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy), 2003