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Enkephalins: Raman spectral analysis and comparison as function of pH 1–13
Author(s) -
Abdali S.,
Refstrup P.,
Nielsen O. Faurskov,
Bohr H.
Publication year - 2003
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10404
Subject(s) - chemistry , raman spectroscopy , molecule , fermi resonance , carboxylate , solvent , raman optical activity , function (biology) , spectral analysis , analytical chemistry (journal) , crystallography , nuclear magnetic resonance , stereochemistry , organic chemistry , spectroscopy , physics , evolutionary biology , optics , biology , quantum mechanics
Raman spectral studies are carried out on Leu‐ and Met‐enkephalin as a function of the pH value in the range of 1–13. The molecules are dissolved in KCl solvent and the pH is controlled at each value. Spectral analyses reveal the dependence of the structural conformation on the pH, and a comparison of the two molecules is made in three frequency regions: the tyrosine Fermi doublet (850–830 cm −1 ), aromatic side chains (1650–1550 cm −1 ), and carboxylate (1430–1400 cm −1 ). All regions and frequencies are presented, discussed, and compared for the two molecules. © 2003 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 72: 318–328, 2003