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Novel fluorescence based receptor binding assay method for receptors lacking ligand conjugates with preserved affinity: Study on estrogen receptor α
Author(s) -
Christoph Silke,
MeyerAlmes FranzJosef
Publication year - 2003
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10402
Subject(s) - chemistry , estrogen receptor , tamoxifen , receptor , ligand binding assay , conjugate , ligand (biochemistry) , estrogen receptor beta , fluorescence anisotropy , radioligand assay , competitive binding , chromatography , biochemistry , breast cancer , cancer , medicine , mathematical analysis , mathematics , membrane
In this study a novel general approach is presented that allows for a straightforward design of receptor binding assays. This principle of a receptor binding assay is applied to the estrogen receptor, which is important in the management of breast cancer and for the estimation of the estrogenic potency of chemicals in the environment. The inhibitory concentrations to reduce cell proliferation in 50% of controls for 17‐β‐estradiol, 4‐hydroxy tamoxifen, and tamoxifen are determined to be 61 n M , 33 n M , and 17 μ M , respectively. The measurement time of the nanoparticle based immunoassay format is 3 s. The Z ′ factor, which is calculated to be 0.89, reflects the excellent assay performance. © 2003 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy), 2003