The thermodynamic contribution of the 5‐methyl group of thymine in the two‐ and three‐stranded complexes formed by poly(dU) and poly(dT) with poly(dA)

To assess the thermodynamic contribution of the 5‐methyl group of thymine, we have studied the two‐stranded helical complexes poly(dA) · poly(dU) and poly(dA) · poly(dT) and the three‐stranded complexes—poly(dA) · 2poly(dU), poly(dA) · poly(dT) · poly(dU) and poly(dA) · 2poly(dT)—by differential scanning calorimetry, and uv optical melting experiments. The thermodynamic quantities associated with the 3 → 2, 2 → 1, and 3 → 1 melting transitions are found to vary with salt concentration and temperature in a more complex manner than commonly believed. The transition temperatures, T m , are generally not linear in the logarithm of concentration or activity of NaCl. The change in enthalpy and in entropy upon melting varies with salt concentration and temperature, and a change in heat capacity accompanies each transition. The poly(dA) · 2poly(dU) triple helix is markedly different from poly(dA) · 2poly(dT) in both its CD spectrum and thermodynamic behavior, while the poly(dA) · poly(dT) · poly(dU) triple helix resembles poly(dA) · 2poly(dT) in these properties. In comparing poly(dA) · 2poly(dT) with either the poly(dA) · poly(dT) · poly(dU) or the poly(dA) · 2poly(dU) triplexes, the substitution of thymine for uracil in the third strand results in an enhancement of stability against the 3 → 2 dissociation of ΔΔ G ° = −135 ± 85 cal (mol A) −1 at 37°C. This represents a doubling of the absolute stability toward dissociation compared to the triplexes with poly(dU) as the third strand. The poly (dA) · poly (dT) duplex is more stable than poly(dA) · poly(dU) by ΔΔ G ° = −350 ± 60 cal (mol base pair) −1 at 37°C. Poly(dA) · poly(dT) has 50% greater stability than poly(dA) · poly(dU) as a result of the dT for dU substitution in the duplex. © 2003 Wiley Periodicals, Inc. Biopolymers 68: 210– 222, 2003