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Importance of minor groove functional groups for the stability of DNA duplexes
Author(s) -
Sun Zhenhua,
Chen Dongli,
Lan Tao,
McLaughlin Larry W.
Publication year - 2002
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10223
Subject(s) - minor groove , chemistry , groove (engineering) , base pair , duplex (building) , minor (academic) , stereochemistry , dna , pyrimidine , oligonucleotide , hydrogen bond , crystallography , biochemistry , molecule , organic chemistry , materials science , political science , law , metallurgy
Eight oligonucleotide duplexes have been prepared with four pairs of selected complementary pairs of native/analogue heterocyclic bases incorporated at a selected test site. The base pairs vary in the nature of their functionality in the minor groove. Each pair has a minor groove purine amino group present or absent, and correspondingly has a minor grove pyrimidine carbonyl present or absent. Loss of duplex stability is most notable when the minor groove pyrimidine carbonyl is absent although in other respects normal Watson–Crick hydrogen bonding is maintained in these sequences. These differences in stability are discussed in terms of possible variations in minor groove hydration. © 2002 Wiley Periodicals, Inc. Biopolymers (Nucleic Acid Sci) 65: 211–217, 2002