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Spin trapping study of reactive oxygen species formation during bopindolol peroxidation
Author(s) -
Kruk Irena,
Michalska Teresa,
Kladna Aleksandra,
AboulEnein Hassan Y.
Publication year - 2002
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10213
Subject(s) - chemistry , singlet oxygen , radical , spin trapping , electron paramagnetic resonance , photochemistry , oxygen , reactive oxygen species , hydroxyl radical , spectrophotometry , organic chemistry , nuclear magnetic resonance , biochemistry , chromatography , physics
The generation of singlet molecular oxygen ( 1 O 2 ) and hydroxyl radicals (HO ·) during peroxidation of bopindolol in the presence of Co(II) ions was studied using electron spin resonance (ESR) and spectrophotometry methods. 2,2,6,6‐Tetramethyl‐4‐piperidone and 5,5‐dimethyl‐1‐pyrroline‐1‐oxide were used as traps. The spectrophotometry determination of 1 O 2 was based on bleaching of p ‐nitrosodimethylaniline (RNO), which was caused by the product of the reaction of 1 O 2 with imidazole and was followed by monitoring the decrease in optical density at 440 nm. The effect of 1 O 2 quenchers and oxygen free radical scavengers on the ESR signal and the bleaching of RNO was studied. The data presented here give new evidence for generation of the reactive oxygen species during peroxidation of bopindolol. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 65: 89–94, 2002