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Surface enhanced IR absorption of nucleic acids from tumor cells: FTIR reflectance study
Author(s) -
Dovbeshko G. I.,
Chegel V. I.,
Gridi. Y.,
Repnytska O. P.,
Shirshov Y. M.,
Tryndiak V. P.,
Todor I. M.,
Solyanik G. I.
Publication year - 2002
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10165
Subject(s) - nucleic acid , chemistry , fourier transform infrared spectroscopy , dna , infrared spectroscopy , substrate (aquarium) , metal , sugar phosphates , phosphate , biophysics , crystallography , analytical chemistry (journal) , biochemistry , chemical engineering , organic chemistry , biology , ecology , engineering
The data on surface enhanced IR absorption (SEIRA) of nucleic acids deposited on a metal substrate were obtained using FTIR in reflectance mode. A 200–400 Å thick gold film on a glass plate was the metal substrate. The approximate enhancement factors of the SEIRA for different vibrations of nucleic acids in our experimental conditions were 3–5. The roughness of the Au surface was about 50 Å. Application of this method to nucleic acids isolated from tumor cells revealed some possible peculiarities of their structural organization, namely, the appearance of unusual sugar and base conformations, modification of the phosphate backbone, redistribution of the H‐bond net, and so forth. This method enhanced a set of the bands, which is impossible to observe in conventional IR geometry. The SEIRA spectra of the RNA from tumor cells showed more sensitivity to the grade of tumor malignancy than the spectra of the DNA. After application of the anticancer drug doxorubicin to sensitive and resistant strains, the DNA isolated from these strains had different spectral features, especially in the region of the phosphate I and II bands. As induced by anticancer drugs, the conformational changes in the DNA from resistant and sensitive cancer strains could be characterized with different levels of structure disordering. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 67: 470–486, 2002