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Anomalously high cooperativity of oligodeoxycytidylic acid for luminescence resonance energy transfer to lanthanide ions
Author(s) -
Lee Jihee,
Park Minyoung,
Son Hyung Su,
Lee Sang Bum,
Lee Hee Cheon,
Ku Ja Kang,
Park Joon Won
Publication year - 2002
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10160
Subject(s) - terbium , lanthanide , luminescence , chemistry , europium , ion , cooperativity , resonance (particle physics) , energy transfer , förster resonance energy transfer , crystallography , photochemistry , atomic physics , chemical physics , fluorescence , materials science , organic chemistry , optoelectronics , optics , biochemistry , physics
The luminescence of terbium(III) and europium(III) through luminescence resonance energy transfer from mononucleotides and oligodeoxynucleotides is examined. Among mononucleotides, dGMP gives the strongest luminescence of terbium(III), while dTMP and dCMP yield a luminescence intensity of europium(III) that is larger than the other two cases. In the homodeoxydecamers, decadeoxycytidylic acid (dC 10 ) produces the highest intensity for both metals. The anomalously large cooperativity of dC 10 is explained by the easiness of deformation of the helical structure to bind lanthanide ions, and a circular dichroism study supports this explanation. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 67: 413–420, 2002