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Studies of low molecular weight samples of glucuronans with various acetylation degree
Author(s) -
PauRoblot Corinne,
Petit Emmanuel,
Sarazin Catherine,
Courtois Josiane,
Courtois Bernard,
Barbotin Jean Noël,
Séguin Jean Paul
Publication year - 2002
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10134
Subject(s) - chemistry , heteronuclear molecule , two dimensional nuclear magnetic resonance spectroscopy , acetylation , intrinsic viscosity , nuclear magnetic resonance spectroscopy , nuclear overhauser effect , fourier transform infrared spectroscopy , carbon 13 nmr , proton nmr , homonuclear molecule , polysaccharide , analytical chemistry (journal) , molecule , polymer , stereochemistry , organic chemistry , biochemistry , physics , quantum mechanics , gene
Partially acetylated, high molecular weight glucuronans were produced by a Sinorhizobium meliloti mutant strain. Two native glucuronan samples with various degrees of acetylation were sonicated to obtain lower molecular weight samples and with low viscosity suitable for chemical modification and 13 C NMR experiments. The average degree of substitution ( DS ) of the polymer was estimated by Fourier transform infrared (FTIR) and NMR. 13 C NMR spectra were obtained and used to suggest a complete assignment of the signals. The nuclear Overhauser effect spectroscopy (NOESY) and heteronuclear multi‐bond coherence (HMBC) experiments were used to elucidate connectivities between the various residues and deduce the linkage of these residues within the polysaccharide. © 2002 Wiley Periodicals, Inc. Biopolymers 64: 34–43, 2002