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Influence of benign cellular changes in diagnosis of cervical cancer using IR microspectroscopy
Author(s) -
Romeo Melissa J.,
Quinn Michael A.,
Burden Frank R.,
McNaughton Don
Publication year - 2002
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10114
Subject(s) - bacterial vaginosis , chemistry , candida albicans , cervical cancer , pathology , cancer , gynecology , medicine , microbiology and biotechnology , biology
IR spectroscopy and principal components analysis (PCA) of endocervical cells and smears diagnosed with benign cellular changes were investigated to determine the influence of these potential confounding variables in the diagnosis of cervical cancer. Spectral differences in all cell and diagnostic types investigated were found in the phosphodiester and carbohydrate regions. However, the spectral differences in other bands were not distinct enough to allow differentiation between groups. The PCA was successfully used to obtain a separation of normal ectocervical smears from normal endocervical cells and smears diagnosed with inflammation, Candida albicans , and bacterial vaginosis. A separation with a slight overlap of abnormal ectocervical smears from normal endocervical cells, inflammation, and bacterial vaginosis was obtained with PCA. Candida was not separated from abnormal ectocervical smears with any success. © 2002 Wiley Periodicals Inc. Biopolymers (Biospectroscopy) 67: 362–366, 2002