Premium
IR spectra and IR spectral maps of individual normal and cancerous cells
Author(s) -
Diem Max,
Chiriboga Luis,
Lasch Peter,
Pacifico Anthony
Publication year - 2002
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10109
Subject(s) - chemistry , nucleic acid , cytoplasm , spectral line , dna , spectral analysis , spectral imaging , resolution (logic) , cell , biophysics , rna , biochemistry , gene , spectroscopy , physics , biology , optics , quantum mechanics , astronomy , artificial intelligence , computer science
The IR microspectra for individual normal and cancerous cells are reported at a spatial resolution that permits a distinction between the nuclear and cytoplasmic regions. The observed spectra reveal large differences in the spectral contributions of RNA, DNA, and phospholipids: metabolically inactive cells show spectral patterns of proteins only, whereas actively dividing cells also show spectral signatures of nucleic acids and phospholipids. These spectral variations are independent of the state of health of a cell. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 67: 349–353, 2002