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Kinetic multichannel spectroscopy of biological molecules: Decomposition of the spectral matrix
Author(s) -
Zimányi László
Publication year - 2002
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10107
Subject(s) - chemistry , bacteriorhodopsin , kinetics , spectral line , molecule , matrix (chemical analysis) , spectroscopy , photochemistry , analytical chemistry (journal) , organic chemistry , biochemistry , physics , chromatography , quantum mechanics , astronomy , membrane
The time‐resolved difference spectra after flash excitation of various biological molecules are measured on a gated optical multichannel analyzer. Electron transfer between the photoactive covalent label thiouredopyrene‐trisulfonate and the heme of cytochrome c and the photocycle of the E204Q mutant bacteriorhodopsin are studied. The spectral matrices containing consecutive difference spectra are analyzed to reveal the reaction kinetics and individual component spectra. Singular value decomposition combined with stoichiometric analysis and self‐modeling is demonstrated as a tool for successful dissection of the matrix, despite the difficulties arising from broad, featureless, overlapping spectra and the overlapping kinetics of the components. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 67: 263–266, 2002