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Study of Langmuir–Blodgett phospholipidic films deposited on surface enhanced Raman scattering active gold nanoparticle monolayers
Author(s) -
Bernard S.,
Felidj N.,
Truong S.,
Peretti P.,
Lévi G.,
Aubard J.
Publication year - 2002
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10086
Subject(s) - monolayer , langmuir–blodgett film , chemistry , raman scattering , surface plasmon resonance , colloidal gold , gold colloid , nanoparticle , raman spectroscopy , analytical chemistry (journal) , adsorption , plasmon , langmuir , photochemistry , nanotechnology , materials science , organic chemistry , optics , optoelectronics , biochemistry , physics
Surface enhanced Raman scattering (SERS) was used to study phospholipid monolayers transferred by the Langmuir–Blodgett (LB) technique to SERS active substrates. These substrates, which were constituted of gold colloidal nanoparticles bound to polysilane films grafted onto glass plates, showed a uniform and homogeneous layer with strong interacting particles as revealed from UV–visible extinction spectra and atomic force microscopy images. Laser excitation at 632.8 nm within the red part of the localized surface plasmon resonance leads to intense and reproducible SERS spectra of trans ‐1,2‐bis(4‐pyridyl)ethylene (BPE). From SERS measurements at different pHs it was possible to determine the apparent p K a of BPE adsorbed on gold‐coated silanized substrates in the absence and presence of one LB monomolecular layer of phospholipids. These SERS titrations allowed the estimation of the pH at the metal–LB film interface. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 67: 314–318, 2002