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Selective monitoring of 3 out of 50,000 protein vibrations
Author(s) -
Barth Andreas
Publication year - 2002
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.10085
Subject(s) - chemistry , phosphate , endoplasmic reticulum , atpase , membrane , biophysics , biochemistry , enzyme , biology
Abstract An important group in a large protein, which is the phosphate group that transiently binds to the Ca 2+ transporting ATPase (Ca 2+ ‐ATPase) of the sarcoplasmic reticulum membrane, is selectively observed with IR spectroscopy. The three phosphate stretching vibrations stand out from the 50,000 protein vibrations in an isotope exchange experiment where oxygen exchange at the phosphate group is catalyzed by the Ca 2+ ‐ATPase. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 67: 237–241, 2002