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Optical properties of ovalbumin in 0.130–2.50 μm spectral region
Author(s) -
Arakawa E. T.,
Tuminello P. S.,
Khare B. N.,
Milham M. E.
Publication year - 2001
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1004
Subject(s) - ovalbumin , chemistry , refractive index , spectral properties , analytical chemistry (journal) , chromatography , optics , computational chemistry , immunology , physics , immune system , biology
In our continuing series of measurements of the complex index of refraction for representative samples of biological materials, we measured ovalbumin (egg albumin) over the spectral region from 0.130 (76,923 cm −1 ) to 2.50 μm (4000 cm −1 ). Films of ovalbumin suitable for optical analyses were prepared and measured in addition to solutions of ovalbumin in water. We show several examples of how the methods used in this study produced accurate results for this complex and difficult to measure material. The present work is applicable to quantitative optical studies involving ovalbumin and other serpin proteins, as well as the study of proteinaceous toxins. © 2001 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 62: 122–128, 2001