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Surface‐enhanced Raman and steady fluorescence study of interaction between antitumoral drug 9‐aminoacridine and trypsin‐like protease related to metastasis processes, guanidinobenzoatase
Author(s) -
Murza Adrian,
SánchezCortés Santiago,
GarcíaRamos José V.
Publication year - 2001
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1001
Subject(s) - chemistry , excimer , fluorescence , raman spectroscopy , fluorescence spectroscopy , monomer , conjugated system , photochemistry , emission spectrum , protease , stereochemistry , enzyme , organic chemistry , spectral line , polymer , physics , quantum mechanics , astronomy , optics
Fluorescence spectroscopy and surface‐enhanced Raman spectroscopy (SERS) were applied to study the interaction of the antitumoral drug 9‐aminoacridine (9AA) with a trypsin‐like protease guanidinobenzoatase (GB) extracted from a mouse Erlich tumor. As a consequence of this interaction, a strong 9AA exciplex emission was detected in the emission fluorescence spectra at certain drug and enzyme concentrations. A SERS study was accomplished on silver colloids at several excitation wavelengths in order to obtain more information about the interaction mechanism. The results derived from Raman spectroscopy indicated that 9AA in the amino monomeric form may interact with the enzyme by means of two different bonds: an ionic bond with a negatively charged amino acid and a ring stacking interaction with an aromatic residue placed in the catalytic site of GB. This interaction mechanism was responsible for a strong exciplex emission detected at a longer wavelength than the expected value of the normal fluorescence emission. Moreover, the GB concentration dependence of the interaction suggested that the drug was sensitive to the quaternary structure of the enzyme. © 2001 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 62: 85–94, 2001