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A novel therapeutic phosphate‐based glass improves full‐thickness wound healing in a rat model
Author(s) -
Taulescu Cristina A.,
Taulescu Marian,
Suciu Maria,
Bolunduț Liviu C.,
Pășcuța Petru,
Toma Corina,
UrdaCîmpean Andrada,
Dreanca Alexandra,
Șenilă Marin,
Cadar Oana,
Ștefan Răzvan
Publication year - 2021
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.202100031
Subject(s) - hacat , biocompatibility , wound healing , in vivo , cytotoxicity , nuclear chemistry , materials science , biomedical engineering , angiogenesis , mtt assay , phosphate , ciprofloxacin , simulated body fluid , viability assay , in vitro , chemistry , surgery , composite material , antibiotics , organic chemistry , medicine , biochemistry , cancer research , metallurgy , scanning electron microscope , microbiology and biotechnology , biology
Wound healing is a highly dynamic process and innovative therapeutic approaches are currently developed to address challenges of providing optimal wound care. In this study, phosphate‐based glasses in the (CuO) x ·(KPO 3 ) 79.5‐x ·(ZnO) 20 ·(Ag 2 O) 0.5 system (CuKPO 3 ZnAg), with different CuO/ KPO 3 ratios were prepared by melt‐quenching technique. Constant Cu concentrations were released from the samples during immersion in Simulated Body Fluid (SBF), while Zn concentrations were slightly decreased over time. Glass surface phosphatation leading to formation of Zn crystalline salts was revealed through spectroscopic techniques. This finding was supported by SEM images that illustrated new compound formation. Subsequent cytotoxicity evaluation on HaCaT Keratinocytes using the indirect MTT cell viability assay revealed a CuO concentration‐dependent cytotoxicity profile and excellent biocompatibility at low CuO concentrations, in all CuKPO 3 ZnAg glasses. Furthermore, the (CuO) 5 ·(KPO 3 ) 74.5 ·(ZnO) 20 ·(Ag 2 O) 0.5 sample (5CuKPO 3 ZnAg) , demonstrated superior antibacterial potency against S. aureus (ATCC 25923) strain compared to amoxicillin and ciprofloxacin. In vivo full‐thickness wound healing evaluation showed a significantly higher regenerative effect of the 5CuKPO 3 ZnAg sample, in terms of angiogenesis, collagen synthesis and re‐epithelialization compared to non‐treated wounds. These findings advance our understanding of the therapeutic perspectives of phosphate‐based glasses, showing promising potential for wound‐healing applications.