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Dissolvable Gelatin‐Based Microcarriers Generated through Droplet Microfluidics for Expansion and Culture of Mesenchymal Stromal Cells
Author(s) -
Ng Ee Xien,
Wang Ming,
Neo Shu Hui,
Tee Ching Ann,
Chen ChiaHung,
Van Vliet Krystyn J.
Publication year - 2021
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.202000048
Subject(s) - microcarrier , mesenchymal stem cell , gelatin , chondrogenesis , microbiology and biotechnology , stromal cell , chemistry , cell , adipogenesis , cell culture , cellular differentiation , bioreactor , cell growth , materials science , biomedical engineering , biology , biochemistry , medicine , cancer research , genetics , organic chemistry , gene
Abstract Microcarriers are synthetic particles used in bioreactor‐based cell manufacturing of anchorage‐dependent cells to promote proliferation at efficient physical volumes, mainly by increasing the surface area‐to‐volume ratio. Mesenchymal stromal cells (MSCs) are adherent cells that are used for numerous clinical trials of autologous and allogeneic cell therapy, thus requiring avenues for large‐scale cell production at efficiently low volumes and cost. Here, a dissolvable gelatin‐based microcarrier is developed for MSC expansion. This novel microcarrier shows comparable cell attachment efficiency and proliferation rate when compared to several commercial microcarriers, but with higher harvesting yield due to the direct dissolution of microcarrier particles and thus reduced cell loss at the cell harvesting step. Furthermore, gene expression and in vitro differentiation suggest that MSCs cultured on gelatin microcarriers maintain trilineage differentiation with similar adipogenic differentiation efficiency and higher chondrogenic and osteogenic differentiation efficiency when compared to MSCs cultured on 2D planar polystyrene tissue culture flask; on the contrary, MSCs cultured on conventional microcarriers appear to be bipotent along osteochondral lineages whereby adipogenic differentiation potential is impeded. These results suggest that these gelatin microcarriers are suitable for MSC culture and expansion, and can also potentially be extended for other types of anchorage‐dependent cells.