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Advances in Lentivirus Purification
Author(s) -
Moreira Ana Sofia,
Cavaco David Guia,
Faria Tiago Q.,
Alves Paula M.,
Carrondo Manuel J. T.,
Peixoto Cristina
Publication year - 2021
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.202000019
Subject(s) - lentivirus , biochemical engineering , process (computing) , computational biology , computer science , suspension culture , cell culture , biology , human immunodeficiency virus (hiv) , engineering , immunology , genetics , viral disease , operating system
Lentiviral vectors (LVs) have been increasingly used as a tool for gene and cell therapies since they can stably integrate the genome in dividing and nondividing cells. LV production and purification processes have evolved substantially over the last decades. However, the increasing demands for higher quantities with more restrictive purity requirements are stimulating the development of novel materials and strategies to supply the market with LV in a cost‐effective manner. A detailed review of each downstream process unit operation is performed, limitations, strengths, and potential outcomes being covered. Currently, the majority of large‐scale LV manufacturing processes are still based on adherent cell culture, although it is known that the industry is migrating fast to suspension cultures. Regarding the purification strategy, it consists of batch chromatography and membrane technology. Nevertheless, new solutions are being created to improve the current production schemes and expand its clinical use.