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Cover Picture: Biotechnology Journal 8/2019
Author(s) -
Duarte Sofia O. D.,
Martins Maria C.,
Andrade Sílvia M.,
Prazeres Duarte M. F.,
Monteiro Gabriel A.
Publication year - 2019
Publication title -
biotechnology journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201970081
Subject(s) - plasmid , lactococcus lactis , escherichia coli , biology , microbiology and biotechnology , genetics , dna , bacteria , gene , lactic acid
Lactococcus lactis is a safer and cost‐effective alternative to the lipopolysaccharide‐producing Escherichia coli as a cell factory for pharmaceutical‐grade plasmid production, but the available plasmids are not able to replicate with a high copy number in L. lactis . The pTRKH3‐b mutant engineered in the present work achieved 215(±38) copies of plasmid per chromosome, a 3.5 fold increase from the non‐modified pTRKH3 (62±5). This is reported by Sofia O. D. Duarte, Maria C. Martins, Sílvia M. Andrade, Duarte M. F. Prazeres, and Gabriel A. Monteiro in article 1800587 .