Premium
Cover Picture: Biotechnology Journal 7/2019
Author(s) -
Jung Yujin,
Lee Chang Y.,
Park Ki S.,
Park Hyun G.
Publication year - 2019
Publication title -
biotechnology journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201970071
Subject(s) - primer (cosmetics) , rnase p , dna , polymerase , primer extension , rnase h , microbiology and biotechnology , taqman , dna polymerase , chemistry , biology , rna , biochemistry , real time polymerase chain reaction , base sequence , gene , organic chemistry
A novel method for sensitive RNase H activity assay by utilizing the target‐activated DNA polymerase activity is described with two main steps, which represents the key concept of our manuscript. In the upper position, the detection probe (Purple strand/Green strand) which binds to and inhibits DNA polymerase (Yellow palm) becomes destabilized by RNase H (Blue palm) that specifi cally degrades the RNA part (Green strand). As a result, the inhibited DNA polymerase recovers its activity and initiates the multiple primer extension reactions in a separate TaqMan probe‐based signal transduction module (Silver strand), leading to the signifi cantly enhanced fl uorescence “turn‐on” signal in the bottom position. This is reported by Yujin Jung, Chang Yeol Lee, Ki Soo Park, and Hyun Gyu Park in the article 1800645 .