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Establishing Suspension Cell Cultures for Improved Manufacturing of Oncolytic Adenovirus
Author(s) -
Moreira Ana Sofia,
Silva Ana Carina,
Sousa Marcos F. Q.,
HagnerMcWhirterc Åsa,
Ahlénc Gustaf,
Lundgren Mats,
Coroadinha Ana S.,
Alves Paula M.,
Peixoto Cristina,
Carrondo Manuel J. T.
Publication year - 2020
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201900411
Subject(s) - oncolytic virus , oncolytic adenovirus , cell culture , bioreactor , suspension culture , virus , virology , chemistry , cancer research , biology , genetics , organic chemistry
Recent clinical trials have shown the potential of oncolytic adenoviruses as a cancer immunotherapy. A successful transition of oncolytic adenovirus to clinical applications requires efficient and good manufacturing practice compatible production and purification bioprocesses. Suspension cultures are preferable for virus production as they can reduce process costs and increase product quality and consistency. This work describes the adaptation of the A549 cell line to suspension culture in serum‐reduced medium validated by oncolytic adenovirus production in stirred tank bioreactor. Cell concentrations up to 3 × 10 6 cells mL −1 are obtained during the production process. At harvest 1.4 × 10 10 infectious particles mL −1 and 6.9 ± 1.1 × 10 10 viral genome mL −1 are obtained corresponding to a viral genome: infectious particles ratio of 5.2 (± 1.9): 1 confirming the virus quality. Overall, the suspension characteristics of these A549 cells support an easily scalable, less time‐consuming, and more cost‐effective process for expanded success in the use of oncolytic viruses for cancer therapy.