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Biotransformation of 5‐Hydroxymethylfurfural to 2,5‐Furandicarboxylic Acid by a Syntrophic Consortium of Engineered Synechococcus elongatus and Pseudomonas putida
Author(s) -
Lin TzuYu,
Wen Rex C.,
Shen Claire R.,
Tsai ShenLong
Publication year - 2020
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201900357
Subject(s) - pseudomonas putida , chemistry , biomass (ecology) , carbon fixation , raw material , biotransformation , yield (engineering) , metabolic engineering , pulp and paper industry , organic chemistry , biology , carbon dioxide , materials science , ecology , engineering , metallurgy , enzyme
Abstract 2,5‐furandicarboxylic acid (FDCA) is one of the top platform chemicals that can be produced from biomass feedstock. To make the cost of industrial FDCA production compatible with plastics made from fossils, the price of substrates and process complexity should be reduced. The aim of this research is to create a CO 2 ‐driven syntrophic consortium for the catalytic conversion of renewable biomass‐derived 5‐hydroxymethylfurfural (HMF) to FDCA. Sucrose produced from carbon fixation by the engineered Synechococcus elongatus serves as the sole carbon source for the engineered Pseudomonas putida to catalyze the reaction of HMF to FDCA. The yield of FDCA by the consortium reaches around 70% while the conversion of HMF is close to 100%. With further surface engineering to clump the two strains, the FDCA yield is elevated to almost 100% via the specific association between an Src homology 3 (SH3) domain and its ligand. The syntrophic consortium successfully demonstrates its green and cost‐effective characteristics for the conversion of CO 2 and biomass into platform chemicals.