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Engineering Tools for the Development of Recombinant Lactic Acid Bacteria
Author(s) -
Cho Sung Won,
Yim Jaewoo,
Seo Sang Woo
Publication year - 2020
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201900344
Subject(s) - microbiology and biotechnology , fermentation , biology , recombinant dna , bacteria , lactic acid , genetically modified organism , genetically engineered , synthetic biology , biochemical engineering , computational biology , food science , gene , biochemistry , genetics , engineering
Abstract Lactic acid bacteria (LAB) is mainly used in food fermentation. In addition, LAB fermentation technology has been studied in the development of industrial food additives, nutrients, or enzymes used in food processing. In the field of red biotechnology, LAB is approved and is generally recognized as a safe organism and is considered safe for biotherapeutic treatments. Recent clinical trials have demonstrated the medicinal value of therapeutic recombinant LAB and the suitability of innate mechanisms of secretion and anchoring for therapeutic applications such as antibody or vaccine production. However, the gram‐positive phenotypic trait of LAB creates challenges for genetic modifications when compared to other conventional workhorse bacteria, resulting in exclusive developments of genetic tools for engineering LAB. In this review, several distinct approaches in gene expression for engineering LAB are discussed.