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Gold Immunochromatography Assay for the Rapid Detection of Spiramycin in Milk and Beef Samples Based on a Monoclonal Antibody
Author(s) -
Guo Lingling,
Liu Liqiang,
Xu Liguang,
Kuang Hua,
Cui Gang,
Xu Chuanlai
Publication year - 2020
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201900224
Subject(s) - spiramycin , chemistry , monoclonal antibody , chromatography , detection limit , antibody , immunoassay , assay sensitivity , microbiology and biotechnology , biology , biochemistry , medicine , antibiotics , erythromycin , immunology , alternative medicine , pathology
Spiramycin (SP) residues in food do harm to human health. It is necessary to establish rapid detection method for SP. In this work, a monoclonal antibody (mAb)‐based gold immunochromatography assay (GICA) is developed for the rapid detection of SP. Under optimum conditions, the half‐maximal inhibitory concentration of SP‐mAb is 0.43 ng mL –1 . The subtype of SP‐mAb is IgG2b. This antibody has no cross‐reactivity with other analogues and has high affinity (4.52 × 10 10 L mol –1 ). Qualitative results can be visualized with the naked eye, with a visual detection limit of 1.0 ng mL –1 and cut‐off value of 10 ng mL –1 . A hand‐held strip scanner is used for the quantitative analysis, with LOD 0.43 ng mL –1 in assay buffer. The recoveries of SP ranged from 72.3% to 112% in milk and 98.5% to 115% in beef, with variable coefficient ranging from 9.4% to 11.7% in milk and 8.14% to 15.4% in beef. Besides, the proposed GICA method for SP is confirmed by LC–MS/MS in SP‐spiked milk and beef samples. Overall, the developed GICA can be a useful tool for SP residues on‐site screening in milk and beef samples.