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Gold Immunochromatographic Assay for Rapid On‐Site Detection of Lincosamide Residues in Milk, Egg, Beef, and Honey Samples
Author(s) -
Guo Lingling,
Wu Xiaoling,
Liu Liqiang,
Kuang Hua,
Xu Chuanlai
Publication year - 2020
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201900174
Subject(s) - lincosamides , lincomycin , chromatography , hapten , chemistry , detection limit , veterinary drug , food science , biology , clindamycin , antibody , biochemistry , antibiotics , immunology
Lincosamides (LMs), include clindamycin (CLIN), lincomycin (LIN), and pirlimycin (PIR), that are widely used as veterinary drugs. LM residues in edible animal origin foods endanger human health and are in urgent need of establishing fast, simple, and highly sensitive detection methods. A gold immunochromatographic strip is prepared to detect CLIN, LIN, and PIR residues simultaneously with a single monoclonal antibody. This antibody is obtained with the design of a novel Hapten and can simultaneously recognize CLIN, LIN, and PIR. Under optimized conditions, the strip results can be semi‐quantitatively evaluated with the naked eye within 15 min, with cut‐off values in phosphate‐buffered saline of 1 ng mL −1 for CLIN, 10 ng mL −1 for LIN, and 25 ng mL −1 for PIR, respectively. Besides, the strip can also be quantified using a hand‐held strip scanner, and the spiked samples are used for establishing matrix curves. The limits of detection for CLIN, LIN, and PIR in spiked milk, egg, beef, and honey samples can satisfy the detection requirement. The utility of this strip is also confirmed by positive honey sample. In short, this strip should be expected to be a useful tool for the rapid on‐site screening of lincosamide residues in milk, egg, beef, and honey samples.