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Cover Picture: Biotechnology Journal 9/2018
Publication year - 2018
Publication title -
biotechnology journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.201870091
Subject(s) - computer science , crispr , cover (algebra) , guide rna , sine qua non , construct (python library) , citation , computational biology , world wide web , microbiology and biotechnology , cas9 , biology , genetics , political science , engineering , programming language , gene , mechanical engineering , law
Metabolic engineering requires an extensive search across the genetic landscape. However, this search (especially in yeast) can be accelerated using new advances in multiplexed dCas9 gene regulation. In article 1700582 , Hal. S. Alper and co‐workers develop a method termed Ligation Extension of gRNA Operons (LEGO) to construct multiplexed gRNA combinations from modular and easily‐synthesized gRNA bricks.